Blog
Live unlock in unicellular functional insights
May
How have single -cell studies evolved over the past two decades?
Over the past 20 years, unicellular studies have evolved from the identification of cell types by means of flow cytometry to discover molecular heterogeneity through single -cell multi -seater, and recently understanding the context of tissues with spatial biology.
Now the field is changing towards the function, revealing what cells actually do, such as the secretion of biomolecules, killing of target cells or interaction with real -time neighbors. This is a new border and Bruker’s cellular analysis is focused there.
Can you introduce a cellular analysis of Bruker and your mission in the space of natural sciences?
During the Bruker cellular analysis, our mission is to enable researchers beyond static cellular identity shutter and dynamic cellular behavior. We focus on the functional analysis of living single cells in real time.
Our platforms, powered by the property of Opoflid technology, help scientists explore multimodal and temporary cellular answers. We support critical applications such as immune profiling, development of cell therapy, discovering antibodies and profiling of antibodies. With the launch of Beacon Discovery™We expand access to these deep insights thanks to the available, compact and profitable platform.
Which inspired the development of Beacon’s discovery™?
Our existing navigation signal® The platforms were designed for large -scale biofarma, but we heard a clear need from researchers in the academic and translational environment: they wanted the same depth of functional view, but in a more flexible reference format.
Discovery of the navigation signal™ It was built to meet this need.
It provides the deepest functional observations from live individual cells, in the footsteps, price and cost of running, which make it widely available. It offers exquisite control, supports non -standard work flows and is equipped with an intuitive interface, making it easier than ever to design and conduct complex functional experiments.
Why is live functional analysis so important for today’s researchers?
Traditional single -cell platforms mainly say what a cell is expressed, such as genes and proteins. But they don’t show you what the cell actually does. In areas such as immunookology, infectious diseases, autoimmune diseaseAnd regenerative medicine, the function matters. Does cell T kill the target? Is it polyfunction? Does cells and secretes a strong, specific antibody? Does the cell actively interact with others or react to the stimulus?
The discovery of Beacon gives scientists tools to ask and answer these questions in real time, launching many sequential tests on the same single cells at many time points. It is not enough for cell examination in a static state. Biology is dynamic and our tools should also be.
What kind of research flow does Beacon Discovery support?
We see exciting work in profiling T cells and the development of cell therapy from the discovery of CAR/TCR to pre -clinical tests and validation for translation biomarker discovery. Scientists carry out many functional tests to characterize cytotoxic activity, secretion of cytokines, activation, exhaustion and durability, all of the same live individual cells, and then following the sequencing of TCR on the same cells.
Beacon’s discovery really shines in discovering antibodies. Scientists can check thousands of B cells from various compartments and species, without mergers, assessing features such as specificity, affinity, cross reactivity, blocking, internalization and agonism. This is a complete, first work flow, which radically shortens the time to run candidates and improves the quality of candidates.
How does Beacon discover this functional view?
It all starts with our opto-electric positioning technology (OEP), which gently moves single live cells to nanoopens on optoselect® token. Then scientists conduct multiplexed, sequential tests, such as cytokine secretion, cell killing or target involvement, in the same cell in time. After identifying the cell of interest, it can be exported to sequencing, combining the function with molecular identity.
In this way, we also provide quick, functional profiling of B cells. In the case of Beacon discovery, researchers receive functional tests of B cells in one day, antibodies sequences during the week and conduct candidates in less than a month, while carrying less unexpressions.
What distinguishes the discovery of Beacon from other platforms to discover antibodies on the market?
The system has three advantages that help to distinguish it from others on the market:
- The width and depth of functional screening tests -We look at various sources of cell B between species and tissues, without the need for cell fusion, and we offer high capacity possibilities.
- The quality of the test matters -Beacon supports multiplexed, multimodal functional tests. This means that you not only identify binders, but you find blockers, agonists and real functional potential customers.
- Efficiency -The platform improves schedules and reduces costs, providing one -day flows of work, full sequences during the week and conducts candidates in less than a month. With fewer false positives, the teams can make faster, more certain decisions of him/no-go.
How do scientists interact with the day system? Is it accessible to non-engineers or non-programmers?
Absolutely. We certainly built Beacon’s discovery. The interface contains a wizard’s style designer, which helps to design multi -storey experiments for several clicks. Our package of mobile analysis software allows for real -time data exploration, image -based analysis and functional mapping such as AMAPS.
Are you a doctorate. A student or experienced immunologist, you can quickly design experiments, analyze data and make decisions. This simplicity was a deliberate design goal.
What is the hoping for Beacon’s discovery in the next few years?
We believe that the discovery of Beacon catalyzes a serious change in the approach of the scientific community of unicellular analysis: from identity to functioning. By making it easier to facilitate the rich, functional tests on live single cells, we help researchers to get better information faster, regardless of whether they identify strong Cells T.Discovering therapeutic antibodies or discovering new biomarkers.
It is a platform that authorizes researchers to experience the full potential of a single -cell functional analysis live, unlocking biological observations, whose static methods cannot reveal.
About the speaker
Dr Vikram Devgan is the vice president of global marketing and product management in BRUREK mobile analysis. Supervises the strategic direction and commercialization of the latest unicellular technologies, including the Beacon® platform. Thanks to his solid experience in the field of marketing and strategy of the product of natural sciences, Vikram consistently emphasizes the integration of functional observations with molecular data in order to increase the progress in therapeutic discovery.
About the Bruker’s cellular analysis
BRURKER cell analysis It authorizes scientists to use the full potential of each cell and manage the next era of functional biology of cells to develop human health. We enable scientists to reveal the most complete information about the function of the cell and obtain a full view of the behavior of each cell.
Our unique package with high bandwidth tools and services offers unparalleled resolution and speed, accelerating observations that are the key to progress in discoveries that can deeply improve the prevention and treatment of the disease. Our award -winning platforms are used by scientists around the world, including those from the 15 best global pharmaceutical companies and about 85% of leading American cancer centers.
