Testing of the brindle Danio reveals the hidden threats associated with environmental toxins from tires

Emerging impurities from N- rubber tires (1.3-dimethylbution) -n’-fenyl-p-fenyleniamine (6PPD) and its 6PPD-kwinone (6PDQ) oxidation product-new red flags for water ecosystems. The recent study reveals that the prolonged exposure to these chemicals at the organic realistic level disturbs the metabolism of lipids and carbohydrates, causes liver damage and changes behavioral patterns in the brindle danio. The study shows that 6PPD accumulates primarily in the liver and 6PPDQ guides the brain. Both compounds are regulated by PPARγ, a key metabolic function regulator, and increase pro -inflammatory cytokines, causing chronic toxicity. In particular, 6PPDQ turned out to be more harmful than its precursor, which suggests that transformation products can be an even greater risk. Discoveries indicate the urgent need for closer regulation and supervision of environmental environment from tires.

N- (1,3-dimethylbutiono) -n’-fenyl-p-fenyleniamine (6PPD), antioxidant widely used in load-bearing tires, plays a key role in preventing rubber degradation under stress. However, after being released to the environment, oxidizes to the 6PPD-quinon complex (6PPDQ), currently located in the world in the waters of rafting and surface. Earlier studies associated both chemicals with developmental and systemic toxicity in aquatic organisms, but the mechanisms lying in their long-term effects on the functioning of the liver and poorly defined neurobehawior-condensible. Danio brindle, due to their genetic similarity to people and suitability for toxicological tests, is a powerful model of tracking these effects. Given the growing fears, a deeper bioacumulation study and chronic toxicity of organs both 6PPD and 6PPDQ were urgently justified.

In the study (DOI: 10.1016/J.ese.2025.100567) published on April 29, 2025, in scientists from the South-Chines of agricultural agricultural universities and colleagues described in detail in detail how the compounds from the tire disturb the liver and neurological functions at Danio Szybki. Thanks to the integration of toxicokinetic tracking, transcript profiling and molecular interaction tests, the team compared the impact of 6PPD and 6PPDQ. Their results revealed that while 6PPD accumulates more in the liver tissue, 6PPDQ causes more serious liver damage. These discoveries emphasize separate toxicity paths and suggest a potential risk that goes beyond fish and other species exposed to chemicals associated with tires in water environments.

In controlled three -month exhibitions, Danio brindle showed clear differences in tissue accumulation and physiological damage. 6PPD was located primarily in the liver, while 6PPDQ was concentrated in the brain. Both compounds impaired the behavior of growth and swimming and caused visible liver abnormalities, such as fattying and degeneration of cells. Enzymatic tests revealed increased levels of markers of hepatic damage (aminotransferase alanine, aspartate aminotransferase, alkaline phosphatase) and oxidative stress indicators (stress emutaz (Supertle, broken, hill) Genes related to the synthesis of lipids, glycolysis and cholesterol regulation. non -alcoholic Fatty liver diseaseIncreasing the ecological and toxicological alarm.

This study emphasizes the hidden threat posed by pollution from rubber during urban rafting. Our findings show that even low, long -term exposure to 6PPD and its oxidation product can seriously interfere with the metabolism and liver behavior in water species. In particular, the 6PPDQ transformation product shows greater toxicity than its precursor, which has significant implications for regulatory monitoring and pollution control. “

Dr. Liangfu Wei, senior author of the study

Dr. Wei emphasized the need to assess environmental risk assessment of both parental compounds and their transformation products in the regulatory assessment.

These results offer key information on environmental risk management and regulatory policy. Identification of PPARγ interference and metabolic disorders is a molecular basis for chronic toxicity supervision. The diversity of 6PPD and 6PPDQ toxic profiles emphasizes the importance of switching on chemical derivatives in the assessment of threats. Discovery requires enhanced inspection of urban rafting and developing advanced water purification systems to reduce water exposure. Considering the protection of metabolic routes on vertebrates, the study also raises wider concerns about the long -term health effects of pollutants from tires to humans through contaminated water sources.